RNA 生物智造技术

原核小干扰 RNA 技术平台

小默生物拥有一种革命性的 RNAi 技术，称为 pro-siRNA（原核小干扰RNA）技术。该技术由小默生物创始人黄林峰教授与美国哈佛医学院 Judy Lieberman 教授（现为小默生物科学顾问）共同发明。一系列相关研究成果已发表在顶级生物技术期刊上，已建立坚实的科学基础。

原核小干扰RNA技术用细菌作为 siRNA 的“生产工厂”，是全球唯一生物工程生产 siRNA 的方法。相比传统的合成工艺例如化学合成，我们的技术实现了最低的生产成本，并通过环境友好型的生物制造工艺实现了超大规模的siRNA生产能力。

此外，原核小干扰RNA技术实现了一种最优的 RNAi 高通量筛选方法，可针对任何物种的任何细胞进行定制化的 RNAi 功能基因筛选。

参考文献

  1. Linfeng Huang, Jingmin Jin, Padraig Deighan, Evgeny Kiner, Larry McReynolds, & Judy Lieberman, Efficient and specific gene knockdown by small interfering RNAs produced in bacteria, Nature Biotechnology, Apr 2013, 31, 350–356 (commented in “Renewable RNAi” of the same issue; highlighted in SciBX, Mar 2013, 6(11)). link to the article
  2. Guneet Kaur, Hung-chi Cheung, Wei Xu, Jun Vic Wong, For Fan Chan, Yingxue Li, Larry McReynolds, & Linfeng Huang, Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli, Biotechnology and Bioengineering, Sep 2018, 115(9):2280-2291. link to the article
  3. Linfeng Huang & Judy Lieberman, Production of highly potent recombinant siRNAs in Escherichia coli, Nature Protocols, Dec 201-3, 8(12), 2325-2336. link to the article
  4. Yingxue Li, Wei Xu, Yutian Ren, Hung-Chi Cheung, Panpan Huang, Guneet Kaur, Chih-Jung Kuo, Sean P. McDonough, Susan L. Fubini, Stephen M. Lipkin, Xin Deng, Yung-Fu Chang*, & Linfeng Huang*, Plakoglobin and High-Mobility Group Box 1 Mediate Intestinal Epithelial Cell Apoptosis Induced by Clostridioides difficile TcdB Clostridioides difficile TcdB, mBio Aug 2022. link to the article
  5. Beibei Hou†, Linhui Qin†, and Linfeng Huang*, Liver cancer cells as the model for developing liver-targeted RNAi therapeutics, Biochemical and Biophysical Research Communications, Feb 2023, 644:85-94. link to the article
  6. Linfeng Huang*, Padraig Deighan, Jingmin Jing, Yingxue Li, Hung-chi Cheung, Elaine Lee, Shirley S. Mo, Heather Hoover, Sahar Abubucker, Nancy Finkel, Larry McReynolds, Ann Hochschild, & Judy Lieberman*, Tombusvirus p19 captures RNase III-cleaved double-stranded RNAs formed by overlapping sense and antisense transcripts in coli, mBio, Jun 2020, 11(3):e00485-20. link to the article

获得授权的专利

  1. ‘Small-interfering RNA expression systems for production of small-interfering RNAs and their use’, Linfeng Huang, Yutian Ren, Hung-chi Cheung, & Guneet Kaur, US patent: 11,193,124
  2. ‘Methods and compositions for the production of siRNAs’, Judy Lieberman & Linfeng Huang, US patent: 9,840,703